EB is based upon an exhaled breath biological sample matrix. A passive collection device containing a thin electret (electrostatic-based) air filter is used to capture submicron bioaerosol particles originating from the airway lining fluid of a donor’s lungs. The single barrel collection device is a one-time-use, low cost and compact unit. Each device is supplied individually boxed in a package containing everything needed for a single sample collection procedure, including a page with clear diagrammatic-based user instructions. The procedure is quick and easy for both the sample Donor and Collector.
See user instructions. (sensabues-user-instructions)
EB has been developed into a versatile platform that offers users a complete solution to collect, extract, detect and identify non-volatile compounds present in exhaled breath. This is a back-to-lab based testing methodology using standard chromatographic-mass spectrometric techniques such as LC-MS/MS, GC-MS/MS and HRMS.
As an integral part of the functional design, a small fraction of the exhaled breath passing through the collection device is diverted into a compact clear plastic bag. This bag serves two functions. Firstly, by inflating, it is a visual indicator that the donor is exhaling their breath through the device correctly. Secondly, when fully inflated, it indicates that the minimum required amount of air (~30 litres) has passed through the filter, which equates to ~3 minutes of normal tidal breathing, or less if the donor prefers to force air through the device. Also, a larger size bag can be used, if a larger sample is required.
The current single-barrel device is suitable for lab-based targeted screening tests. A double-barrelled device suitable for A+B sample confirmatory tests, is in design.
Sensabues has filed applications for, and been granted, patents for key design features and functional aspects of the EB sampling device, system and method.
More information on this technology and product is available to download from the RESOURCES page on this website, including a list of the published technical papers and a list of FAQs.